First of all, Trypsin-EDTA is a product for freezing, so it is important not to take too long to thaw.
It is recommended to melt at 37 degrees and use it quickly as soon as it melts, and thawing at 37 degrees for a long time may reduce the performance of the product.
In addition, when stored at a temperature higher than the storage temperature, it can affect the performance of the product
Because trypsin-EDTA is a protein, storage at high temperatures can degrade or degrade its activity.
Therefore, it is recommended to store trypsin-EDTA in a refrigerated (-20°C to -5°C) or frozen (-20°C).

It can also be used as our product FBS, and in general, FBS is added at a concentration of 10%, but please note that the optimal concentration may vary depending on the cell line. Viruses may also have optimal concentrations of FBS that can affect virus proliferation, so we recommend testing samples first.

In the case of our product BCS (Ironfortified) products, it is recommended because it is a product that reinforces iron in general BCS and has better performance and is not difficult to use when there is no problem in use.

No, we are not going through any additional filters.
If the filter process affects the use of the product
It can be delivered considering including it in the manufacturing process separately.
First of all, I recommend using the sample.

Fetal Bovine Serum (FBS) is used to promote cell growth and survival in cell culture
It is a nutritional source of cell culture.
BCS (Bovine Calf Serum) is used for relatively less differentiated cells or specific experimental conditions,
It is suitable for slow growth or cell maintenance due to low growth factors.
In other words, it provides an environment suitable for slow growth conditions or specific experiments.
In summary, FBS is used to promote cell growth,
BCS is for slow growth or special conditions experiments.

There may be performance differences depending on the origin of FBS (Fetal Bovine Serum), but it is not simply due to the origin, but because it is a product derived from animal serum due to the nature of FBS, the variation between Lot is expected to be large even within the product of the same origin.
However, since subtle differences may be important depending on the purpose of the study or cell type, it is recommended to use it after sample testing and reference in advance.

HBSS and EBSS are isotonic buffers for osmotic and pH maintenance, used for short-term cell maintenance.
HBSS can be used in general atmospheric environments without CO₂ incubators due to its low NaHCO ₃ content, and EBSS is designed to maintain pH through HCO ₃⁻/CO ₃² ⁻ systems within CO₂ incubators due to its high NaHCO ₃ content.

Both PBS and DPBS play essential roles in biological and biochemical studies. However, while PBS is a common buffer solution with its simple composition, DPBS provides an enhanced formulation specifically tailored to cell culture.
In addition, DPBS can be divided into calcium and magnesium versions, and D-glucose and pyruvate, so it is recommended to choose and use them according to the purpose of the experiment.

Defrosting cells quickly is the most important thing.
Defrosting usually takes place quickly at 37°C, and it is recommended that the cells be adapted to the culture environment as soon as possible.

After thawing, appropriate media should be used to prevent rapid pH changes or damage to the medium components, and an environment to culture the cells should be prepared.

To increase cell viability after thawing, replace new medium as soon as possible.

DMSO(Dimethyl Sulfoxide) plays a very important role in cell freezing, but in the case of high concentrations of DMSO(Dimethyl Sulfoxide) , it can cause toxicity to cells.
It is important to remove DMSO(Dimethyl Sulfoxide) by transferring cells to the culture medium as soon as possible upon thawing after freezing.